Home 
General Physiology and Biophysics 2012 General Physiology and Biophysics Vol.31, No.1, p.11–18, 2012
General Physiology and Biophysics 2012 General Physiology and Biophysics Vol.31, No.1, p.11–18, 2012
Journal info
Aims and Scope |
||
Select Journal
Journals
Acta Virologica Bratislava Medical Journal Ekologia - Ecology Endocrine Regulations General Physiology and Biophysics Ahead of print 2013 2012 2011 2010 2009 2008 2007 2005 Neoplasma Studia Psychologica Životné prostredie Psychológia a patopsych. dieťaťa Building Research Journal Metallic Materials Slovenská hudba Musicologica SlovacaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
General Physiology and Biophysics Vol.31, No.1, p.11–18, 2012 |
||
| Title: Changes in cellular response to the damage induced in PC-3 prostate cancer cells by proton microbeam irradiation | ||
| Author: Ewelina W. Lipiec, Anna Wiecheć, Joanna Dulińska-Litewka, Małgorzata Kubica, Janusz Lekki, Zbigniew Stachura, Joanna Wiltowska-Zuber, Wojciech M. Kwiatek | ||
| Abstract: The aim of this research was to find out whether the passage number effect may influence on the PC-3 cells (the human prostate cancer line derived from bone metastases) response to proton radiation. 2 MeV horizontally focused proton microbeam was used as a radiation source. The cells were treated with a counted number of H+ ions (50–8000) corresponding to doses of 1.3–209 Gy/cell. For comparison, cell death was also induced by UVC radiation. All cells were stained with Hoechst 33342 and propidium iodide and visualized under a fluorescence microscope. Necrosis was observed at: a) 8000 protons per cell (corresponding to ~209 Gy/cell) after 2–4 passages, b) 3200 protons per cell (corresponding to ~84 Gy/cell) for cells after 11–14 passages and c) only 800 protons per cell (corresponding to ~2 Gy/cell ) after 47–50 passages. Apoptosis was efficiently induced, by protons, only in cells after 50 passages. The results showed that the laboratory conditions affected cellular response of PC-3 cell line to the proton irradiation. The cellular response to the radiation treatment strongly depends on number of passages. |
||
| Keywords: PC–3 cells — Single cell irradiation — Cellular response — Proton microprobe | ||
| Published online: 08-Feb-2011 | ||
| Year: 2012, Volume: 31, Issue: 1 | Page From: 11, Page To: 18 | |
| doi:10.4149/gpb_2012_001 |
||
|
Price:
14.40 €
|
||
|
|
||