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CUSTOMERS General Physiology and Biophysics 2012 General Physiology and Biophysics Vol.31, No.1, p.11–18, 2012
CUSTOMERS General Physiology and Biophysics 2012 General Physiology and Biophysics Vol.31, No.1, p.11–18, 2012
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General Physiology and Biophysics Vol.31, No.1, p.11–18, 2012 |
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| Title: Changes in cellular response to the damage induced in PC-3 prostate cancer cells by proton microbeam irradiation | ||
| Author: Ewelina W. Lipiec, Anna Wiecheć, Joanna Dulińska-Litewka, Małgorzata Kubica, Janusz Lekki, Zbigniew Stachura, Joanna Wiltowska-Zuber, Wojciech M. Kwiatek | ||
| Abstract: The aim of this research was to find out whether the passage number effect may influence on the PC-3 cells (the human prostate cancer line derived from bone metastases) response to proton radiation. 2 MeV horizontally focused proton microbeam was used as a radiation source. The cells were treated with a counted number of H+ ions (50–8000) corresponding to doses of 1.3–209 Gy/cell. For comparison, cell death was also induced by UVC radiation. All cells were stained with Hoechst 33342 and propidium iodide and visualized under a fluorescence microscope. Necrosis was observed at: a) 8000 protons per cell (corresponding to ~209 Gy/cell) after 2–4 passages, b) 3200 protons per cell (corresponding to ~84 Gy/cell) for cells after 11–14 passages and c) only 800 protons per cell (corresponding to ~2 Gy/cell ) after 47–50 passages. Apoptosis was efficiently induced, by protons, only in cells after 50 passages. The results showed that the laboratory conditions affected cellular response of PC-3 cell line to the proton irradiation. The cellular response to the radiation treatment strongly depends on number of passages. |
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| Keywords: PC–3 cells — Single cell irradiation — Cellular response — Proton microprobe | ||
| Published online: 08-Feb-2011 | ||
| Year: 2012, Volume: 31, Issue: 1 | Page From: 11, Page To: 18 | |
| doi:10.4149/gpb_2012_001 |
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