Home HOME Acta Virologica 2012 Acta Virologica Vol.56, No.1, p.25-30, 2012

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.56, No.1, p.25-30, 2012

Title: A set of host proteins interacting with papaya ringspot virus NIa-Pro protein identified in a yeast two-hybrid system
Author: L. GAO, W. T. SHEN, P. YAN, D. C. TUO, X. Y. LI, P. ZHOU

Abstract: The protein-protein interactions between viral and host proteins play an essential role in plant virus infection and host defense. The potyviral nuclear inclusion protein a protease (NIa-Pro) is involved in various steps of viral infection. In this study, the host proteins interacting with papaya ringspot virus (PRSV) NIa-Pro were screened in a Carica papaya L. plant cDNA library using a Sos recruitment two-hybrid system (SRS). We confirmed that the full-length EIF3G, FBPA1, FK506BP, GTPBP, MSRB1, and MTL from papaya can interact specifically with PRSV NIa-Pro in yeast, respectively. These proteins fufill important functions in plant protein translation, biotic and abiotic stress, energy metabolism and signal transduction. In this paper, we discuss possible functions of interactions between these host proteins and NIa-Pro in PRSV infection and their role in host defense.

Keywords: Sos recruitment two-hybrid system; papaya ringspot virus; NIa-Pro; protein-protein interaction
Year: 2012, Volume: 56, Issue: 1 Page From: 25, Page To: 30
doi:10.4149/av_2012_01_25
Price: 12.00 €






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