Home HOME Acta Virologica 2012 Acta Virologica Vol.56, No.1, p.71-74, 2012

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.56, No.1, p.71-74, 2012

Title: A rapid and highly reliable field-based LAMP assay of canine parvovirus

Abstract: Loop-mediated isothermal amplification (LAMP) is known as a rapid and reliable alternative to conventional single-step or nested PCR for detection of genomic DNA of various pathogens in clinical samples. In this study, LAMP assay was developed for canine parvovirus (CPV) and compared with single-step and nested PCR assays. Out of 50 fecal samples from dogs clinically suspected for CPV infections, 19 were found positive by single-step PCR, 22 by nested PCR and 26 by LAMP. LAMP products were subjected to restriction analysis and sequencing to check their specificity. LAMP assay turned out to be a rapid and fairly reproducible method, did not amplify other common canine pathogens and was more sensitive than nested PCR assay. Therefore, it can be regarded as a highly reliable method for routine field diagnosis of CPV infection.

Keywords: canine parvovirus; nested polymerase chain reaction; loop-mediated isothermal amplification; sensitivity; specificity
Year: 2012, Volume: 56, Issue: 1 Page From: 71, Page To: 74
Price: 8.00 €

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