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General Physiology and Biophysics Vol.31, No.2, p.225–227, 2012 |
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Title: Study of interaction of long-chain n-alcohols with fluid DOPC bilayers by a lateral pressure sensitive fluorescence probe | ||
Author: Tatiana N. Murugova, Mária Klacsová, Petra Pullmannová, Janka Karlovská, Pavol Balgavý | ||
Abstract: Abstract. The excimer 1,2-dipyrenedecanoyl-sn-glycero-3-phosphatidylcholine (dipy10PC) fluorescence probe was used to determine effects of aliphatic alcohols (CnH2n+1OH, n = 12–18 is the even number of carbons in alkyl chain) on fluid dioleoylphosphatidylcholine (DOPC) + dioleoylphosphatidylserine (DOPS) bilayers in multilamellar vesicles at molar ratio DOPC/DOPS = 24.7. The excimer to monomer fluorescence intensity ratio increases with the increase of CnH2n+1OH/DOPC molar ratio and decreases with the CnH2n+1OH alkyl chain length n at a constant CnH2n+1OH/DOPC = 0.4 molar ratio. These effects indicate changes in the bilayer lateral pressure on the level of pyrenyl moieties location. |
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Keywords: Lipid bilayer — Dioleoylphosphatidylcholine — Alkan-1-ol — Dipyrenylphosphatidylcholine | ||
Year: 2012, Volume: 31, Issue: 2 | Page From: 225, Page To: 227 | |
doi:10.4149/gpb_2012_021 |
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