Neoplasma Vol.59, No.6, p.676-684, 2012

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Neoplasma Vol.59, No.6, p.676-684, 2012
Title: Enhanced chemosensitivity to CPT-11 in colorectal carcinoma xenografts by small hairpin RNA interference targeting PLK1
Author: P. FAN, S. ZHANG, H. TIAN, N. YAN, L. DAI, X. ZHANG, L. CHENG, C. LI, Y. LI, X. CHEN, G . SHI, Y . YANG, Y. WEI, H. DENG

Abstract: Commonly used drugs for the treatment of colon{} cancer patients like CPT-11 shows severe side effects or induces resistance in clinical settings. Thus, we analyzed a combination of PLK1 (polo-like kinase 1)-specific short hair RNA (shRNA), a potent tool to destroy mitosis in cancer cells, together with CPT-11 to enhance drug sensitivity. Cellular proliferation and apoptosis were determined in SW620 colorectal carcinoma cells. Knockdown of cellular PLK1 led to the decreased mRNA and PLK1 protein in RT-PCR and western blot assay. The viability declined (pnohistochemistry, accompanied with TUNEL assay. As we expect, the combination treatment delayed tumor growth (p of PLK1-specific shRNA interference with low-dose CPT-11 triggered a antitumor efficacy and represented a potential strategy to treat colon cancer.

Keywords: CPT-11, drug resistance, SN-38, PLK1, RNA interference, colorectal carcinoma
	Published online: 01-Aug-2012
Year: 2012, Volume: 59, Issue: 6	Page From: 676, Page To: 684
doi:10.4149/neo_2012_086
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