Home Acta Virologica 2012 Acta Virologica Vol.56, No.3, p.199-207, 2012

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.56, No.3, p.199-207, 2012

Title: Identification of cellular proteins interacting with influenza A virus PB1-F2 protein
Author: Z. H. GUAN, M. L. ZHANG, P. L. HOU, M. DUAN, Y. M. CUI, X. R. WANG

Abstract: The influenza A virus (IAV) protein PB1-F2, which is encoded by an alternative ORF of the PB1 polymerase gene, has been implicated as an important virulence factor and apoptosis inducer. However, the molecular mechanism of PB1-F2 function remains elusive. In this study, eight cellular proteins were identified as potential PB1-F2 interacting partners using the yeast two-hybrid system. Two positive candidate proteins, guanine nucleotide binding protein (G protein) beta polypeptide 2 (Gβ2) and macrophage migration inhibitory factor (MIF), were selected to be further characterized. The interaction of MIF and Gβ2 with PB1-F2 was confirmed by both GST pull-down and co-immunoprecipitation assays. Confocal laser microscopy showed that the interaction between PB1-F2 and the two cellular proteins occurred in the cytoplasm. The novel interactions between PB1-F2 and host proteins provide further pieces of evidence in the investigation of the pathogenic mechanism of IAV.

Keywords: influenza A virus; PB1-F2; yeast two-hybrid; protein-protein interaction
Year: 2012, Volume: 56, Issue: 3 Page From: 199, Page To: 207

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