General Physiology and Biophysics Vol.32, No.2, p.215-219, 2

General Physiology and Biophysics Vol.32, No.2, p.215-219, 2013

Founded: 1982
ISSN 1338-4325 (online)
ISSN 0231-5882 (print)
Published in English,
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General Physiology and Biophysics Vol.32, No.2, p.215-219, 2013
Title: Polyanion-induced circular dichroism of Thioflavin T
Author: Diana Fedunova, Peter Huba, Jaroslava Bagelova, Marian Antalik

Abstract: Thioflavin T (ThT) is amyloid specific fluorescence dye possessing the properties of molecular rotor. We have shown that Thioflavin T forms complexes with non-peptide polyanions heparin, polyadenylate and polystyrene sulphonate by means of absorption spectroscopy. In the presence of chiral polyanions – heparin and polyadenylate, induced optical activity of ThT occurs whereas interaction with achiral polystyrene sulphonate (PSS) does not lead to production of induced circular dichroism signal. The positively charged ThT forms centre for ordered binding of chiral polyanion. Similarly, complexation of structurally different chromophore 9-aminoacridine with polyanions has led to induction of optical activity only in the presence of chiral ones. We suggest that, primarily, the optical activity of environment plays important role in inducing optical activity of achiral compounds.

Keywords: Thioflavin T — Induced circular dichroism — Polyanion
	Published online: 24-Jan-2013
Year: 2013, Volume: 32, Issue: 2	Page From: 215, Page To: 219
doi:10.4149/gpb_2013020
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