Home FOR AUTHORS General Physiology and Biophysics 2013 General Physiology and Biophysics Vol.32, No.2, p.251–259, 2013

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ISSN 0231-5882 (print)
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General Physiology and Biophysics Vol.32, No.2, p.251–259, 2013

Title: The long-term administration of Orai 1 antagonist possesses antitussive, bronchodilatory and anti-inflammatory effects in experimental asthma model
Author: Martina Šutovská, Michaela Kocmálová, Marian Adamkov, Desanka Výbohová, Pavol Mikolka, Daniela Mokrá, Jozef Hatok, Martina Antošová, Soňa Fraňová
Abstract: The best-studied store-operated Ca2+ channels (SOCs), Ca2+ release activated Ca2+ (CRAC) channels, are activated by depleting endoplasmic reticulum (ER) Ca2+ pool and mediate Ca2+ influx vitally important for Ca2+ restoration and many cellular function. CRAC channels were identified on immune and airway smooth muscle (ASM) cells. Emerging evidence points to its involvement in allergic airways diseases. This article evaluated therapeutic potency of CRAC antagonist in experimental animal model of allergic asthma. Allergic asthma, induced by repetitive exposure of guinea pigs to ovalbumine, was followed by 14 days therapy by CRAC antagonist (3-fluoropyridine-4-carboxylic acid, FPCA). In vivo changes of specific airways resistance (sRaw) evaluated bronchodilatory effect of FPCA and salbutamol. The method of citric acid-induced cough reflex assessed antitussive activity of FPCA and codeine. The measurement of exhaled NO (ENO), expression of inducible NO synthase (iNOS) by RT-PCR and immunohistochemical staining of airways tissue verified anti-inflammatory effect of FPCA. Long-term administration of FPCA resulted in significant cough suppression and bronchodilation, both comparable to the effect of control drugs. FPCA significantly decreased ENO and iNOS expression, which together with mmunohistochemical analysis validated its anti-inflammatory effect. Presented data confirmed CRAC channels as a promising target for treatment of respiratory diseases associated with allergic inflammation.
Keywords: Mesenchymal stem cells in prostate cancer have higher expressions of SDF-1, CXCR4 and VEGF
Published online: 24-Jan-2013
Year: 2013, Volume: 32, Issue: 2 Page From: 251, Page To: 259
doi:10.4149/gpb_2013018


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