Home FOR AUTHORS General Physiology and Biophysics 2013 General Physiology and Biophysics Vol.32, No.2, p.277–283, 2013

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General Physiology and Biophysics Vol.32, No.2, p.277–283, 2013

Title: Effects of caloric restriction on oxidative stress parameters
Author: Marija Stankovic, Dusan Mladenovic, Milica Ninkovic, Danijela Vucevic, Tina Tomasevic, Tatjana Radosavljevic
Abstract: Moderate caloric restriction prolongs lifespan. Changes in oxidative stress (OS) and hormesis may be involved in this process. The aim of this study is to examine the effects of different levels of chronic caloric restriction (CR) and acute fasting on stress response and OS parameters in rat liver and plasma. Forty-two rats were divided into groups: control group, calorie-restricted groups with intake of 80–90%, 60–70%, 40–50%, 20–30% of daily caloric needs (DCN) and acute fasting group (AF). To determine alanine aminotransferase (ALT), aspartate aminotransferase (AST) and superoxide dismutase (SOD) activity, concentration of corticosterone, nitrites and nitrates (NOx), malondialdehyde (MDA) and glutathione (GSH), liver samples and blood were collected. Increase in plasma corticosterone concentration and AST and ALT activity was found in severe CR. Ingestion 40–50% DCN or less increased liver MDA and NOx concentration and decreased SOD activity. Ingestion 60–70% DCN increased Mn-SOD activity, GSH and NOx. In AF and group taking 20–30% DCN, GSH was significantly lower than in control group. Severe CR and acute fasting increase oxidative damage and decrease antioxidative capacity of hepatocytes. Moderate CR increases antioxidative capacity of hepatocytes due to increase in Mn-SOD activity and GSH concentration, which might have a role in anti-aging and hormetic mechanism of CR.
Keywords: Caloric restriction — Oxidative stress — Hormesis — Acute fasting
Published online: 26-Feb-2013
Year: 2013, Volume: 32, Issue: 2 Page From: 277, Page To: 283
doi:10.4149/gpb_2013027


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