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General Physiology and Biophysics 2013 General Physiology and Biophysics Vol.32, No.1, p.67–78, 2013
General Physiology and Biophysics 2013 General Physiology and Biophysics Vol.32, No.1, p.67–78, 2013
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General Physiology and Biophysics Vol.32, No.1, p.67–78, 2013 |
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| Title: A viscometric approach of pH effect on hydrodynamic properties of human serum albumin in the normal form | ||
| Author: Karol Monkos | ||
| Abstract: The paper presents the results of viscosity determinations on aqueous solutions of human serum albumin (HSA) at isoelectric point over a wide range of concentrations and at temperatures ranging from 5°C to 45°C. On the basis of a modified Arrhenius equation and Mooney’s formula some hydrodynamic parameters were obtained. They are compared with those previously obtained for HSA in solutions at neutral pH. The activation energy and entropy of viscous flow and the intrinsic viscosity reach a maximum value, and the effective specific volume, the self-crowding factor and the Huggins coefficient a minimum value in solutions at isoelectric point. Using the dimensionless parameter [η]c, the existence of three ranges of concentrations: diluted, semi-diluted and concentrated, was shown. By applying Lefebvre’s relation for the relative viscosity in the semi-dilute regime, the Mark-Houvink-Kuhn-Sakurada (MHKS) exponent was established. The analysis of the results obtained from the three ranges of concentrations showed that both conformation and stiffness of HSA molecules in solutions at isoelectric point and at neutral pH are the same. |
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| Keywords: Human serum albumin — Activation energy — Effective specific volume — Intrinsic viscosity — Huggins coefficient — Mark-Houvink-Kuhn-Sakurada exponent | ||
| Year: 2013, Volume: 32, Issue: 1 | Page From: 67, Page To: 78 | |
| doi:10.4149/gpb_2013011 |
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