Home FOR AUTHORS Neoplasma 2013 Neoplasma Vol.60, No.5, p.504-510, 2013

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.60, No.5, p.504-510, 2013

Title: Establishment of an animal model of spontaneous cervical lymph node metastasis of laryngeal squamous cell carcinoma and obtaining laryngocarcinoma cells with high metastatic potential
Author: L. W. CHEN, J. L. WANG, L. Y. ZHANG, S. M. YANG, C. S. LI, N. YU, J. D. ZHAO,W. W. GUO, L. D. ZHAO, K. LI, M. B. LIU, S. Q. ZHAI

Abstract: To establish an animal model of spontaneous cervical lymph node metastasis of laryngeal squamous cell carcinoma and obtain laryngocarcinoma cells with high metastatic potential, laryngeal squamous cell carcinoma cell line HEP-2 in logarithmic phase were inoculated under the lingual margin mucosa of nude mice. HEP-2 cells metastasized to the cervical lymph nodes were isolated, cultured, and re-inoculated under the lingual margin mucosa of nude mice twice. The tumor formation in the tongue and in the cervical lymph nodes was confirmed by pathological examination. Carcinoma cells’ ability of invasion and migration was detected by transwell assay. Human specific Alu sequences were detected by PCR, which indicated that the tumor cells originated from human laryngeal squamous cell carcinoma cell line HEP-2. Finally, an animal model of spontaneous lymph node metastasis of laryngeal squamous cell carcinoma was successfully established. Laryngeal squamous cell carcinoma cells with high metastatic potential to lymph nodes were obtained through repeated inoculations.

Keywords: laryngeal cancer, lymph node metastasis, animal model
Published online: 24-Jun-2013
Year: 2013, Volume: 60, Issue: 5 Page From: 504, Page To: 510
doi:10.4149/neo_2013_065


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