Home CUSTOMERS Neoplasma 2013 Neoplasma Vol.60, No.6, p.627-634, 2013

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ISSN 0028-2685
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Neoplasma Vol.60, No.6, p.627-634, 2013

Title: Proteasome inhibition leads to altered signaling in the proteome of cisplatin-resistant human ovarian carcinoma cell line
Author: J. DURAJ, M. PASTOREK, J. VITKOVSKA, D. CHOLUJOVA, P. GRONESOVA, L. HUNAKOVA, J. SEDLAK

Abstract: To address a precise view into molecular mechanisms of apoptotic signaling pathways after single- or combinatory treatments with specific NF-κB- or proteasome inhibitors and/or cisplatin (CDDP), flow cytometry and western blotting of the cell proteome in human ovarian chemosensitive- and CDDP-resistant cell lines were used. We report here that proteasome inhibition (but not NF-κB inhibition) caused marked alterations in the cell proliferation and cell cycle, as well as in the levels of signaling anti- and pro-apoptotic proteins PARP, NF-κB, IκB-α, Bcl-2, Bax, and lysosome-associated LAMP-1 and ATP-7B molecules in particular proteome fractions. These findings refer to the possibility of regulation of CDDP resistance, inclusive the capacity of lysosomes to export CDDP in certain human ovarian cancer cells by proteasome inhibition.

Keywords: cisplatin resistance, cell proteome, NF-κB- and proteosome inhibition, flow cytometry, western blotting
Published online: 01-Aug-2013
Year: 2013, Volume: 60, Issue: 6 Page From: 627, Page To: 634
doi:10.4149/neo_2013_081


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