Home HOME General Physiology and Biophysics 2013 General Physiology and Biophysics Vol.32, No.3, p.311–323, 2013

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Founded: 1982
ISSN  1338-4325 (online)

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General Physiology and Biophysics Vol.32, No.3, p.311–323, 2013

Title: Isoproterenol accelerates apoptosis through the over-expression of the sodium/calcium exchanger in HeLa cells
Author: Sona Hudecova, Lubomira Lencesova, Lucia Csaderova, Jan Sedlak, Viera Bohacova, Marcela Laukova, Olga Krizanova


Apoptosis induction causes over-expression of the Na+/Ca2+ exchanger of type 1 (NCX1) in the HeLa cell line. During induction of apoptosis and in the presence of isoproterenol hydrochloride (I; β-adrenergic agonist), increase in the NCX1 is even more pronounced. Anti-apoptotic Bcl-2 mRNA and protein is markedly reduced during apoptosis and in the presence of I, which causes a rapid increase in the Bax/Bcl-2 ratio. During apoptosis induction by apoptosis inducing kit (A), both with and without I, the active form of caspase-3, which is the executive enzyme in apoptosis, becomes visible on Western blots.

Silencing NCX1 resulted in the reversal of the Bax/Bcl-2 ratio, it prevented a decrease in mitochondrial membrane potential compared to the AI group and it decreased the level of AI-induced apoptosis in HeLa cells. Based on the experiments with single apoptotic inducers camptothecin, cycloheximide and dexamethasone, it might be proposed that potentiated apoptotic effect in I-treated cells is due to the inhibition of nuclear topoisomerase. As illustrated in immunofluorescence and Western blot analysis, calnexin increased significantly during induction of the apoptosis in the presence of I. In addition, further decrease in sarco/endoplasmic ATPase 2 (SERCA2), decrease in reticular calcium and mitochondrial membrane potential was observed, which suggests development of the endoplasmic reticulum (ER) stress. Based on these results, we propose that I further enhanced NCX1 expression in apoptotic cells through the development of ER stress.

Keywords: Na+/Ca2+ exchanger — Apoptosis — Isoproterenol hydrochloride — Endoplasmic reticulum stress
Year: 2013, Volume: 32, Issue: 3 Page From: 311, Page To: 323

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