Home Endocrine Regulations 2013 Endocrine Regulations Vol.47, No.3, p.205-209, 2013

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Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

Published in English

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Endocrine Regulations Vol.47, No.3, p.205-209, 2013

Title: Effects of retinoic acid isomers on proteomic pattern in human breast cancer MCF-7 cell line
Author: D. Flodrova, D. Benkovska, D. Macejova, L. Bialesova, J. Bobalova, J. Brtko

Abstract: Retinoids, acting through their cognate nuclear receptors, are crucial transcriptional regulators of many cellular processes such as differentiation, development, apoptosis, carbohydrate and lipid metabolism, homeostasis, etc. The aim of this study was the exploration of molecular mechanisms in relation to therapy of human breast cancer. One of the efficient strategies is identification of biomarkers as important tools in early cancer diagnosis and advisable treatment. Retinoids have been regarded as important therapeutic agents for many types of cancers, including human breast cancer. The effects of all-trans retinoic acid and 9-cis retinoic acid or their combination on proteomic pattern in human MCF-7 breast cancer line were investigated. The total cell proteins were extracted utilizing a commercially Radio-Immunoprecipitation Assay (RIPA) buffer and separated on 1D sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE). The proteins were subsequently digested in-gel by trypsin and identified by matrix assisted laser desorption ionization technique with time of flight mass analyzer (MALDI-TOF/TOF). Our data offer novel information on the proteomic pattern of proteins evaluated after treatment of MCF-7 cells with retinoic acid isomers.

Keywords: retinoic acid isomers, retinoid, breast cancer, malignant cells, proteomic analysis
Year: 2013, Volume: 47, Issue: 4 Page From: 205, Page To: 209
Price: 10.00 €

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