Home Acta Virologica 2013 Acta Virologica Vol.57, No.4, p.429-434, 2013

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.57, No.4, p.429-434, 2013

Title: Expression of bovine Mx1 protein inhibits the replication of foot-and-mouth disease virus in BHK-21 cells
Author: K. J. CAI, Q. L. MENG, J. QIAO, J. HUANG, Z. C. ZHANG, G. C. WANG, J. W. WANG, C. F. CHEN

Abstract: Mx proteins belonging to the dynamin superfamily of large GTPases inhibit replication of a wide range of RNA viruses. In this study, we examined whether bovine Mx1 protein could interfere with the replication of foot-and-mouth disease virus (FMDV). For this purpose we established cloned BHK-21 cells expressing bovine Mx1 protein (BM1 cells) and infected them with FMDV serotype O. Cloned BHK-21 cells expressing neomycin resistance instead of Mx1 protein (BH1 cells) and original BHK-21 cells served as negative controls. The results showed that the expression of bovine Mx1 protein reduced viral yields by 90% and levels of viral VP1 mRNA by 60%. These findings correlated with a significant reduction of viral antigen detectable in infected cells by immunofluorescent assay. These results demonstrate that bovine Mx1 protein interferes with the replication of FMDV.

Keywords: foot-and-mouth disease virus; Mx1 protein; BHK-21 cells
Year: 2013, Volume: 57, Issue: 4 Page From: 429, Page To: 434
doi:10.4149/av_2013_04_429


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