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General Physiology and Biophysics Vol.33, No.1, p.131–136, 2014 |
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Title: Effect of Pb2+ ions on photosynthetic apparatus | ||
Author: Frantisek Sersen, Katarina Kralova, Matus Pesko, Marek Cigan | ||
Abstract: Using model lead compounds Pb(NO3)2 and Pb(CH3CHOO)2 the mechanism and the site of action of Pb2+ ions in the photosynthetic apparatus of spinach chloroplasts were studied. Both compounds inhibited photosynthetic electron transport (PET) through photosystem 1 (PS1) and photosystem 2 (PS2), while Pb(NO3)2 was found to be more effective PET inhibitor. Using EPR spectroscopy the following sites of Pb2+ action in the photosynthetic apparatus were determined: the water-splitting complex and the Z•/D• intermediates on the donor side of PS2 and probably also the ferredoxin on the acceptor side of PS1, because cyclic electron flow in chloroplasts was impaired by treatment with Pb2+ ions. Study of chlorophyll fluorescence in suspension of spinach chloroplasts in the presence of Pb2+ ions confirmed their site of action in PS2. Using fluorescence spectroscopy, also formation of complexes between Pb2+ and amino acid residues in photosynthetic proteins was confirmed and constants of complex formation among Pb2+ and aromatic amino acids were calculated for both studied lead compounds. |
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Keywords: EPR — Fluorescence spectroscopy — Lead(II) acetate — Lead(II) nitrate — Photosynthetic electron transport | ||
Year: 2014, Volume: 33, Issue: 1 | Page From: 131, Page To: 136 | |
doi:10.4149/gpb_2013074 |
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