Home 
General Physiology and Biophysics 2014 General Physiology and Biophysics Vol.33, No.1, p.137–144, 2014
General Physiology and Biophysics 2014 General Physiology and Biophysics Vol.33, No.1, p.137–144, 2014
Journal info
Aims and Scope |
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
General Physiology and Biophysics Vol.33, No.1, p.137–144, 2014 |
||
| Title: Triptolide induces apoptosis through the SERCA 3 upregulation in PC12 cells | ||
| Author: Olga Krizanova, Jana Markova, Karel Pacak, Ludovit Skultety, Andrea Soltysova, Sona Hudecova | ||
| Abstract: Diterpenoid triepoxide – Triptolide (TTL) – increased protein levels of the noradrenaline transporter in three pheochromocytoma cell lines. This transporter is involved in the apoptosis induction through the inhibition of a transcription factor NF-kappa B. Nevertheless, calcium release from the endoplasmic reticulum can also induce inner, mitochondrial pathway of apoptosis in variety of cells. Therefore, the aim of this work was to evaluate an involvement of calcium and, more specifically, intracellular calcium transport systems in the apoptosis induction in pheochrocytoma cell line PC12. We observed significantly increased amount of reticular calcium in TTL-treated cells compared to control, untreated cells. Surprisingly, gene expression of the IP3 receptors was not changed after the TTL treatment, but ryanodine receptor of the type 2 (RyR2) was downregulated and sarco/endoplasmic reticulum calcium ATPase type 3 (SERCA 3) was upregulated in TTL- treated cells, compared to untreated controls. SERCA 3 blocking with the specific blocker thapsigargin prevented increase in apoptosis observed by the TTL treatment. Decrease in the ATP production by a replacement of glucose in the cultivation medium for its non-utilizable analog 2-deoxyglucose also prevented induction of the apoptosis in TTL-treated PC12 cells. Thus, these results suggest that upregulation of the SERCA 3 is ultimately involved in the TTL-induced apoptosis in PC12 cells. |
||
| Keywords: Triptolide — Calcium transport — SERCA 3 — Apoptosis | ||
| Year: 2014, Volume: 33, Issue: 1 | Page From: 137, Page To: 144 | |
| doi:10.4149/gpb_2014004 |
||
|
|
 download file |
|