Home Acta Virologica 2014 Acta Virologica Vol.58, No.1, p.34-42, 2014

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ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.58, No.1, p.34-42, 2014

Title: Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India
Author: B. BABU, V. HEGDE

Abstract: Dasheen mosaic virus (DsMV) infecting three major edible aroids namely Amorphophallus paeoniifolius, Colocasia esculenta, and Xanthosoma sagittifolium cultivated in India was characterized. Infected plants showing typical DsMV symptoms were subjected to reverse transcription-polymerase chain reaction, and an amplification of a 963 bp fragment which encoded the coat protein (CP) gene was obtained. BLAST analysis of the cloned DNA amplicon revealed the identity of the virus to be that of DsMV. Sequence identity matrix of the nucleotide sequences among the three isolates showed that the DsMV isolate infecting A. paeoniifolius and C. esculenta shared an identity as high as 93%, while the DsMV isolate from X. sagittifolium shared an identity of only 73% and 76% with the DsMV isolates from A. paeoniifolius and C. esculenta, respectively. Comparative analysis of the coat protein of the three DsMV isolates showed the presence of DVG motif (A. paeoniifolius and C. esculenta) and DTG motif in X. sagittifolium and several varying potential threonine and asparagine rich N-glycosylation motifs. Single amino acid substitution of the several conserved motifs occurs in all the three DsMV isolates. This is the first characterization of DsMV isolates infecting A. paeoniifolius, C. esculenta, and X. sagittifolium plants in India.

Keywords: Amorphophallus paeoniifolius; coat protein; Colocasia esculenta; potyvirus; RT-PCR; Xanthosoma sagittifolium
Published online: 10-Apr-2014
Year: 2014, Volume: 58, Issue: 1 Page From: 34, Page To: 42
doi:10.4149/av_2014_01_34


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