Home Bratislava Medical Journal 2014 Bratislava Medical Journal Vol.115, No.4, p.203-208, 2014

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2

 

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Bratislava Medical Journal Vol.115, No.4, p.203-208, 2014

Title: Phosphoinositid signal pathway mediate neurite outgrowth in PC12 cells by staurosporine
Author: H. Zhaleh, M. Azadbakht, A. Bidmeshki Pour

Abstract: Objectives: In previous studies, we showed that staurosporine uses intracellular calcium ions to affect cell death in PC12 cells. The bulk release of intracellular excessive Ca2+ from intracellular sources into cytosol contributes to neuronal apoptotic events, which in turn results in neuronal cell death. However, the mechanisms of Ca2+-induced neuronal cell death or neurite elongation is still unclear. Therefore, we investigated the relation between phosphoinositid signal pathway, intracellular calcium, and reactive oxygen species on one hand, with staurosporine-induced neurite outgrowth in PC12 cells on the other.
Results: The inhibition of phospholipase C or IP3 receptor antagonist or phosphoinositid signal transduction antagonist produced cell death and suppressed neurite outgrowth by staurosporine in PC12 cells. The inhibition of these enzymes and pathway results in an increase in intracellular Ca2+ although subsequent hydroxyl radical (•OH) production began after inhibitors exposure. •OH production was significantly attenuated in inhibitor supplemented medium treatment, and it was dependent on the intracellular Ca2+ concentration. These data indicate that staurosporine activates phosphoinositid signal pathway while endoplasmic Ca2+, and subsequent •OH production are critical events in staurosporine-induced neurite outgrowth in PC12 cells.
Conclusion: We conclude that the fact that staurosporine mobilizes Ca2+, probably via activating the subcellular compartment, is responsible for staurosporine-induced (Ca2+]i increase during neurite outgrowth in PC12 cells (Fig. 7, Ref. 30). T

Keywords: endoplasmic reticulum, neurite outgrowth, staurosporine, PC12 cells.
Year: 2014, Volume: 115, Issue: 4 Page From: 203, Page To: 208
doi:10.4149/BLL_2014_043


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