Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
General Physiology and Biophysics Vol.33, No.2, p.145–155, 2014 |
||
Title: Potent cough suppression by physiologically active substance in human plasma | ||
Author: Norio Akaike, Yushi Ito, Sachie K. Ogawa, Megumi Maeda, Masahito Wakita, Kazuo Takahama, Tetsuro Noguchi, Shintaro Kamei, Takayoshi Hamamoto, Misako Umehashi, Hiroaki Maeda | ||
Abstract: Human plasma contains wide variety of bioactive proteins that have proved essential in therapeutic discovery. However, many human plasma proteins remain orphans with unknown biological functions. Evidences suggest that some plasma components target the respiratory system. In the present study we adapted heparin affinity chromatography to fractionate human plasma for functional bioassay. Fractions from pooled human plasma yielded particular plasma fractions with strong cough suppressing effects. Purification yielded a fraction that was finally identified as an activated blood coagulation factor XIa (fXIa) using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI/TOF-MS). The fraction almost completely suppressed coughs induced by either chemical or mechanical stimulation applied to larynx or bifurcation of guinea-pig trachea. Cough suppressing effect of the fraction and commercially available fXIa were one million times stronger than codeine and codeine only partially suppressed the mechanically triggered coughing in animal model. Recent reviews highlighted prominent shortcomings of current available antitussives, including narcotic opioids such as codeine and their unpleasant or intolerable side effects. Therefore, safer and more effective cough suppressants would be welcome, and present findings indicate that fXIa in human plasma as a very promising, new therapeutic candidate for effective antitussive action. |
||
Keywords: Human plasma — Cough suppressing factor — Factor XIa | ||
Year: 2014, Volume: 33, Issue: 2 | Page From: 145, Page To: 155 | |
doi:10.4149/gpb_2013083 |
||
|
download file |
|