Home Acta Virologica 2014 Acta Virologica Vol.58, No.2 p.137-145, 2014

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.58, No.2 p.137-145, 2014

Title: Detection and molecular characterization of Egyptian isolates of grapevine viruses
Author: F. FATTOUH, C. RATTI, A.M.D. EL-AHWANY, E. ABDEL ALEEM, A. R. BABINI, C. RUBIES AUTONELL

Abstract: Selected commercial and/or local vineyards and nurseries in three different governorates of Egypt (Alexandria, El-Beheira and El-Menofia) were surveyed for symptoms indicative of infection by grapevine viruses. Leaf samples from red-fruited and white-fruited Vitis vinefera were tested for grapevine leafroll associated viruses (GLRaV-1, GLRaV-2, and GLRaV-3), grapevine viruses A and B (GVA, GVB), grapevine rupestris stem pitting virus (GRSPaV), grapevine fanleaf virus (GFLV), and grapevine fleck virus (GFKV) from early April to late October 2010. Incidence of these viruses was assessed by RT-PCR in 60 different samples. Selected amplicons were sequenced. While GVA was the most wide spread (30%), GLRaV-1, GVB, GFLV, and GFKV were not detected during the survey. However, GVA, GLRaV-2, GLRaV-3, and GRSPaV were detected in the form of single infection or in mixed infections of 2 to 4 viruses. Phylogenetic analysis was performed on all Egyptian isolates of GLRaV-2 (4), GLRaV-3 (7), GVA (3), and GRSPaV (6). GRSPaV was detected for the first time in Egypt. Phylogenetic analysis provided insights into the evolutionary relationship between the reported Egyptian isolates and other previously reported isolates.

Keywords: Egypt; grapevine viruses; ELISA; RT-PCR; detection; sequence analysis
Year: 2014, Volume: 58, Issue: 2 Page From: 137, Page To: 145
doi:10.4149/av_2014_02_137


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