Home Bratislava Medical Journal 2014 Bratislava Medical Journal Vol.115, No.7, p.427-433, 2014

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2

 

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Bratislava Medical Journal Vol.115, No.7, p.427-433, 2014

Title: Paeoniflorin inhibited the tumor invasion and metastasis in human hepatocellular carcinoma cells
Author: J. T. Lu, W. He, S. S. Song, W. Wei

Abstract: Objective: Evidence suggests that paeoniflorin may be involved in anticancer activities. Here, we have investigated the effects of paeoniflorin and correlative mechanisms on anti-invasion and anti-metastasis in human hepatocellular carcinoma (HCC) cell lines.
Materials and methods: In the current study, we have applied 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay to determine the proliferative effect of HepG2 and Bel-7402, two human hepatoma cell lines, and we have established a boyden chamber assay, a wound healing assay and cell adhesion assay to detect and quantify the invasion, metastasis and adhesion of both HepG2 and Bel-7402. In addition, we have analyzed the protein expression of matrix metalloproteinas (MMP)-9, E-cadherin (E-cad) and extracellular signal-regulated kinase (ERK) in both cell lines through western blot analysis.
Results: Paeoniflorin (6. 25–200 µM) had inhibitory effect on the growth of HepG2 and Bel-7402 cell lines, and reduced significantly invasion, metastasis and adhesion of HCC cell lines. In addition, paeoniflorin decreased the expression of MMP-9 and ERK in HepG2 and Bel-7402 cells, and increased expression of E-cad in both cell lines.
Conclusions: Paeoniflorin is effective anti-metastatic and anti-invasive agent for suppressing HCC invasion and metastasis (Fig. 5, Ref. 30).

Keywords: paeoniflorin, hepatocellular carcinoma, anti-invasion, anti-metastasis.
Year: 2014, Volume: 115, Issue: 7 Page From: 427, Page To: 433
doi:10.4149/BLL_2014_084


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