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Acta Virologica Vol.58, No.3 p.214-222, 2014 |
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Title: Molecular characterization of barley yellow dwarf virus in Tunisia | ||
Author: M. BOUALLEGUE, M. MEZGHANI-KHEMAKHEM, D. BOUKTILA, H. MAKNI, M. MAKNI | ||
Abstract: Barley yellow dwarf disease is a worldwide ubiquitous virus disease of cereal crops. In order to characterize the B/CYDV isolates occurring in Tunisia, 240 barley leaves were randomly sampled from 6 fields following a North-South trend and analyzed by serological and molecular tests. DAS-ELISA results showed 40 positive samples with a prevalence of barley yellow dwarf virus (BYDV)-PAV (77.5%), followed by cereal yellow dwarf virus (CYDV)-RPV (25%) and BYDV-MAV (15%). Studies of the geographic distribution showed a high incidence of B/CYDV in the Tunisian Southern provinces. RT-PCR assays were performed to amplify the viral coat protein gene (CP) and sequence analyses revealed six BYDV-PAV haplotypes named PAV-TN1 to PAV-TN6. Phylogenetic analysis showed that the six Tunisian haplotypes were close to BYDV-PAV-II subspecies and had a strong similarity with Moroccan, Czech, French and German haplotypes. Although PAV-TN2 and PAV-TN5 showed up to 10% divergence from BYDV-PAV-II at the amino acid level, it seems to belong to the same subspecies but in a separated cluster. Our results will be important in developing appropriate control measures against BYDV disease in Tunisia. |
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Keywords: barley yellow dwarf virus; cereal yellow dwarf virus; DAS-ELISA; CP gene; RT-PCR; PAV-II | ||
Year: 2014, Volume: 58, Issue: 3 | Page From: 214, Page To: 222 | |
doi:10.4149/av_2014_03_214 |
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