Home Bratislava Medical Journal 2014 Bratislava Medical Journal Vol.115, No.9, p.532-537, 2014

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564


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Bratislava Medical Journal Vol.115, No.9, p.532-537, 2014

Title: Effect of interleukin 12 (IL-12) on embryonic development and yolk sac vascularisation
Author: M. Nisari, H. Ulger, E. Unur, O. Karaca, T. Ertekin

Abstract:  Background: In the recent days there has been an increase in diseases known as “angiogenic diseases” characterized by pathologic vascularisation. In the rat, the development of embryonic vessel starts to occur at 9.5 days of gestation. In mammals, the vascular system starts developing in a very early embryonic stage. The majority of rat embryo circulation system gets complete approximately at 11 – 12 days. Therefore the in vitro study of 9.5 – 11.5-day old embryo culture could be a suitable model to study the effects of angiogenic and antiangiogenic substances on yolk sac vascularisation. In the present study, the effects of Interleukin-12 (IL-12) on the yolk sac vascularisation are investigated during the in vitro embryo culture, where the latter angiogenic factor was added to serum.
Methods: After 48-hour culture period, effects of different doses of IL-12 (50 ng/ml, 100 ng/ml, and 200 ng/ml) were estimated morphologically.
Results: According to morphologic scoring system, the total morphologic score, yolk sac diameter, crown rump length, and somite number were retarded in all experimental groups when compared to control. These developmental retardations were statically significant. There was also a poor development in the yolk sac vascularisation and the heart.
Conclusion: As a result, the IL-12 could cause developmental retardation of embryos owing to its antiangiogenic effect (Tab. 3, Fig. 2, Ref. 39).

Keywords: interleukin 12, vascularisation, yolk sac, embryo culture.
Year: 2014, Volume: 115, Issue: 9 Page From: 532, Page To: 537

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