Home FOR AUTHORS Neoplasma 2015 Neoplasma Vol.62, No.1, p.81-87, 2015

Journal info


6 times a year.
Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

Published in English

Editorial Info
Abstracted and Indexed
Submission Guidelines

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Neoplasma Vol.62, No.1, p.81-87, 2015

Title: Overexpression of Survivin mutant Thr34Ala induces apoptosis and inhibits gastric cancer growth
Author: S. C. Dang, S. Feng, P. J. Wang, L. Cui, J. G. Qu, J. X. Zhang

Abstract: Survivin is highly expressed in human gastric cancer and correlated with chemoresistance and poor prognosis. In this study, we explored the effect of adeno-associated virus (AAV)-mediated survivin dominant mutant Thr34Ala (rAAV-Sur-Mut(T34A)) on gastric cancer growth. AAV-Sur-Mut (T34A) virus was generated and purified using the AAV vector pAM/CAG-WPRE.poly(A) vector. Cell proliferation was determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) Assay. Apoptosis was determined by Flow cytometry and Terminal deoxynucleotidyl transferase dUTP nick end labeling. Gene expression was determined by Western blot and immunohistochemistry. Tumor growth was evaluated using a xenograft mouse model. Overexpression of survivin promoted cell growth of gastric cancer cells. Infection of rAAV-Sur-Mut(T34A) virus inhibited cell proliferation, induced apoptosis and sensitized gastric cancer cells to 5-Fluorouracil in vitro. Treatment of rAAV-Sur-Mut(T34A) significantly inhibited cell proliferation, induced apoptosis and inhibited gastric cancer growth in vivo. Our results suggest that the combination of rAAV-Sur-Mut(T34A) and chemotherapy may be a new approach for gastric cancer therapy.

Keywords: Survivin, survivin mutant T34A, adeno-associated virus, gastric cancer, apoptosis, gene therapy
Published online: 17-Oct-2014
Year: 2015, Volume: 62, Issue: 1 Page From: 81, Page To: 87
doi:10.4149/neo_2015_010


download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.