Home Acta Virologica 2014 Acta Virologica Vol.58, No.4, p.333-339, 2014

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.58, No.4, p.333-339, 2014

Title: Recombinant VP1 protein of duck hepatitis virus 1 expressed in Pichia pastoris and its immunogenicity in ducks
Author: C. WANG, X.K. LI, T. C. WU, Y. WANG, C. J. ZHANG, X. C. CHENG, P. Y. CHEN

Abstract: The VP1 gene of duck hepatitis virus type 1 (DHV-1) strain VJ09 was amplified by reverse transcription PCR from the liver of a duckling with clinical symptoms of viral hepatitis. The resulting VP1 cDNA was 720 bp in length and encoded a 240-amino-acid protein. In VP1 gene-based phylogenetic analysis, the VJ09 strain grouped with DHV-1 genotype C. The VP1 gene was inserted into the expression vector pPICZαA and expressed in Pichia pastoris. The expressed VP1 protein was purified and identified by western blot analysis. To evaluate the recombinant VP1’s immunogenic potential in ducklings, the antibodies raised in the immunized ducklings were titrated by ELISA, and lymphocyte proliferation and virus neutralization assays were performed. The results show that the recombinant VP1 protein induced a significant immune response in ducklings and this could be a candidate for the development of a subunit vaccine against DHV-1 genotype C.

Keywords: duck hepatitis virus 1; genotype C; VP1 protein; Pichia pastoris; duck; immunogenicity
Year: 2014, Volume: 58, Issue: 4 Page From: 333, Page To: 339
doi:10.4149/av_2014_04_333


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