Journal info
Aims and Scope |
||
Select Journal
Journals
Acta Virologica Current articles 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Acta Virologica Vol.58, No.4, p.333-339, 2014 |
||
Title: Recombinant VP1 protein of duck hepatitis virus 1 expressed in Pichia pastoris and its immunogenicity in ducks | ||
Author: C. WANG, X.K. LI, T. C. WU, Y. WANG, C. J. ZHANG, X. C. CHENG, P. Y. CHEN | ||
Abstract: The VP1 gene of duck hepatitis virus type 1 (DHV-1) strain VJ09 was amplified by reverse transcription PCR from the liver of a duckling with clinical symptoms of viral hepatitis. The resulting VP1 cDNA was 720 bp in length and encoded a 240-amino-acid protein. In VP1 gene-based phylogenetic analysis, the VJ09 strain grouped with DHV-1 genotype C. The VP1 gene was inserted into the expression vector pPICZαA and expressed in Pichia pastoris. The expressed VP1 protein was purified and identified by western blot analysis. To evaluate the recombinant VP1’s immunogenic potential in ducklings, the antibodies raised in the immunized ducklings were titrated by ELISA, and lymphocyte proliferation and virus neutralization assays were performed. The results show that the recombinant VP1 protein induced a significant immune response in ducklings and this could be a candidate for the development of a subunit vaccine against DHV-1 genotype C. |
||
Keywords: duck hepatitis virus 1; genotype C; VP1 protein; Pichia pastoris; duck; immunogenicity | ||
Year: 2014, Volume: 58, Issue: 4 | Page From: 333, Page To: 339 | |
doi:10.4149/av_2014_04_333 |
||
|
![]() |
|