Home HOME Neoplasma 2015 Neoplasma Vol.62, No.3, p.384-390, 2015

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.62, No.3, p.384-390, 2015

Title: miR-29b inhibits the progression of esophageal squamous cell carcinoma by targeting MMP-2
Author: Y. QI, X. LI, S. ZHAO


Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human esophageal cancer and crucial to tumorigenesis. Herein, we identified the role of miR-29b in esophageal squamous cell carcinoma (ESCC) development in vitro and in vivo. MiR-29b expression was investigated in thirty cases of ESCC samples as well as four ESCC cell lines by real-time PCR. Transwell assays were conducted to explore the effects of miR-29b on the invasion of human ESCC cell lines. The impact of overexpression of miR-29b on putative target MMP-2 were subsequently confirmed via Western blot.

Our results indicated that MiR-29b expression was frequently down-regulated in ESCC specimens and cell lines compared with adjacent normal tissues (p<0.05).

Overexpression of miR-29b suppressed (p<0.05) ESCC cell invasion, as well as the growth of xenograft tumors in mice.

Overexpression of miR-29b significantly decreased (p<0.05) the protein level of MMP-2, which has previously been identified as a direct target of miR-29b.

Thus, our study demonstrated that overexpression of miR-29b inhibits tumor growth in part by targeting MMP-2. Our findings revealed that miR-29b may act as a tumor suppressor in ESCC, whose dysregulation may be involved in the initiation and development of human ESCC.

Keywords: MiR-29b, esophageal squamous cell carcinoma, invasion, MMP-2
Published online: 01-Apr-2015
Year: 2015, Volume: 62, Issue: 3 Page From: 384, Page To: 390

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