Acta Virologica Vol.59, No.1, p.20-26, 2015

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.59, No.1, p.20-26, 2015
Title: Tandem overexpression of five human factors renders murine hepatocytes susceptible to hepatitis C virus
Author: L. LV, Q. KANG, X. YU, B. GAO, T. HU, P. MA, Y. ZHANG, F. YAN, J. XIAO, J. DENG, X. ZHOU, J. XU

Abstract: Development of mouse model of hepatitis C virus (HCV) infection has great significance in drug screening and vaccine research. The barriers of interspecies transmission of HCV are increasingly better understood. Human factors, namely low-density lipoprotein receptor (hLDLR), CD81 (hCD81), scavenger receptor class B type I (hSCARB1), occludin (hOCLN) and claudin 1 (hCLDN1) are all required for rendering mouse hepatocytes permissive to HCV. With the aim to humanize mouse hepatocytes we constructed two recombinant vectors tandemly expressing the first three and the last two HCV entry factors mentioned above, respectively. Cotransfection of mouse hepatocytes with these vectors made them permissive to HCV binding and entry. Tandem overexpression of hLDLR, hSCARB1, hCD81, hCLDN1 and hOCLN is a novel approach to tailoring mouse hepatocytes to HCV binding and entry which can be further used to establish a mouse model of HCV infection as a basis for developing antiviral drugs and vaccines.

Keywords: hepatitis C virus; mouse hepatocytes; human factors

Year: 2015, Volume: 59, Issue: 1	Page From: 20, Page To: 26
doi:10.4149/av_2015_01_20
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