Home HOME Acta Virologica 2015 Acta Virologica Vol.59, No.1, p.84-91, 2015

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.59, No.1, p.84-91, 2015

Title: Immunoreactivity and trypsin sensitivity of recombinant virus-like particles of foot-and-mouth disease virus
Author: S. H. BASAGOUDANAVAR, M. HOSAMANI, R. P. TAMIL SELVAN,P. SARAVANAN, B. P. SREENIVASA, B. K. CHANDRASEKHAR SAGAR, R. VENKATARAMANAN

Abstract: Foot-and-mouth disease (FMD) is an important infection affecting the health and productivity of cloven-hoofed livestock. Development of improved vaccines and diagnostic reagents is being explored to facilitate the disease control. There is an emerging interest in virus-like particles (VLPs), as their constituent structural proteins are the major immunogens. The VLPs are similar to natural virus particles but lack viral nucleic acid. The objective of the present study was to express the VLPs of FMD virus (FMDV) serotype Asia-1 (IND 63/72), using baculovirus system and characterize them for antigenic structure. The VLPs expressed in insect cells showed immunoreactivity similar to inactivated cell culture FMDV. Further they possess similar sensitivity to trypsin as the inactivated cell culture FMDV, suggesting that trypsin-sensitive antigenic sites could be similarly arranged. Our findings suggest that the FMD VLPs have similar antigenic conformational feature like the wild type virus, thus supporting their utility in development of non-infectious FMD vaccines and/or diagnostic assays.


Keywords: foot-and-mouth disease virus; virus-like particle; bacmid; recombinant baculovirus; trypsin sensitivity; immunoreactivity
Year: 2015, Volume: 59, Issue: 1 Page From: 84, Page To: 91
doi:10.4149/av_2015_01_84


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