Acta Virologica Vol.59, No.1, p.92-97, 2015

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.59, No.1, p.92-97, 2015
Title: Self-priming on the plant viral RNAs during reverse transcription-PCR
Author: C. ZHANG, H. N. WU, Y. Q. ZHANG, J. G. SHEN, W. M. LI

Abstract: The occurrence of the primer-independent cDNA synthesis during RT-PCR analysis of human and animal RNA viruses has been well documented. Conversely, there is scant knowledge about this event in plant RNA viruses. Here we show that the primer-independent cDNA synthesis occurs in all eight different plant RNA viruses tested in this study, suggesting a common phenomenon for RT-PCR analysis of plant RNA viruses. Additional experiments indicate that the event is likely contributed to by RNA self-priming, and can be effectively reduced or eliminated through increasing temperature of the RT reaction.

Keywords: RT-PCR; primer-independent cDNA synthesis; self-priming; plant RNA virus; RT temperature

Year: 2015, Volume: 59, Issue: 1	Page From: 92, Page To: 97
doi:10.4149/av_2015_01_92
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