Home Acta Virologica 2015 Acta Virologica Vol.59, No.2, p.148-155, 2015

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.59, No.2, p.148-155, 2015

Title: Induction of interferon lambda in influenza a virus infected cells treated with shRNAs against M1 transcript
Author: P. ŠVANČAROVÁ, D. SVETLÍKOVÁ, T. BETÁKOVÁ

Abstract:

RNA interference (RNAi) represents a form of post-transcriptional gene silencing mediated by small interfering RNAs (siRNA) and provides a powerful tool to specifically inhibit viral infection. To investigate therapeutic capacity of siRNAs targeting M gene, six vectors with U1-short hairpin RNA (shRNA) expression system were prepared and tested in infected cells and animals.

In infected cells, three of six shRNAs targeting M1 gene significantly (P <0,01) reduced the virus titer to 66%, 45% or 21%, respectively.

Replication of IAV and levels of M1 RNAs were significantly reduced in the cells transfected with shRNAs, which decreased the virus titer. IFN-α/β altered in shRNAs-treated cells. The level of IFN-λ (type III interferon) mRNA was significantly increased in the infected cells treated with shM22, shM349, shM522, and (type I interferon) as well as IP-10 (type II interferon) mRNAs were not significantly their mixtures. The increased level of IFN-λ mRNA corresponded to significantly increased level of RIG-1 mRNA. shRNAs inhibited influenza virus infection in a gene-specific manner in co-operation with IFN-λ. Some constructs targeting the M1 transcript prolonged the survival of infected mice.



Keywords: influenza virus; shRNA; M1 protein
Published online: 22-Jun-2015
Year: 2015, Volume: 59, Issue: 2 Page From: 148, Page To: 155
doi:10.4149/av_2015_02_148


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