Home Acta Virologica 2015 Acta Virologica Vol.59, No.2, p.156-165, 2015

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Acta Virologica Vol.59, No.2, p.156-165, 2015

Title: Pathotyping of recent Indian field isolates of Marek's disease virus serotype 1
Author: P. SURESH, J. JOHNSON RAJESWAR, K. SUKUMAR, T. J. HARIKRISHNAN, P. SRINIVASAN

Abstract:

A study was undertaken to assess the virulence of Marek's disease virus (MDV) serotype 1 field isolates obtained from poultry flocks of southern part of India. Five representative MDV serotype 1 strains were isolated from eighty-six blood samples collected from fifteen farms. Three out of five isolates which were free from avian leukosis virus (ALV) and reticuloendotheliosis virus (REV) were adapted in chicken embryo fibroblast (CEF) culture and designated as Ind/TN/11/01, Ind/KA/12/02 and Ind/TN/12/03. Pathotyping assay was conducted in two trials. In the first trial, non-vaccinated chickens were challenged (trial I), while in second trial, two types of vaccinated chickens along with non-vaccinated controls were challenged (trial II).

Birds inoculated with field isolate Ind/TN/12/03 had very low body (75.34±3.04 g 15 days post infection (dpi)) and bursa Fabricii weight (1.64±0.06 at 15 dpi) when compared to those inoculated with the other two isolates (Ind/TN/11/01 and Ind/KA/12/02) and uninoculated controls (body weight 111.33±1.30 g and bursa Fabricii weight 4.33±0.11 15 dpi).

Incidence of early mortality syndrome (53%) and lymphoma (86%) induced by Ind/TN/12/03 was comparable with very virulent strains published elsewhere. In protection test, the percentage of Marek's disease (MD) incidence induced by Ind/TN/12/03 was 57.5% and 25% in monovalent and bivalent vaccine inoculated birds respectively compared to uninoculated control (100%). Based on the above findings in pathotyping experimental trials with a supportive evidence of histopathological observations, isolate Ind/TN/12/03 was considered as very virulent MDV and other two isolates were considered as virulent MDVs.



Keywords: pathotypes of Marek's disease virus; incidence of lymphoma, early mortality syndrome; HVT and SB1
Published online: 22-Jun-2015
Year: 2015, Volume: 59, Issue: 2 Page From: 156, Page To: 165
doi:10.4149/av_2015_02_156


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