Home General Physiology and Biophysics 2015 General Physiology and Biophysics Vol.34, No.3, p.331–334, 2015

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Quarterly, 80 pp. per issue
Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.34, No.3, p.331–334, 2015

Title: Agonistic effect of selected isoflavones on arylhydrocarbon receptor in a novel AZ-AhR transgenic gene reporter human cell line
Author: Lucia Bialesova, Aneta Novotna, Dana Macejova, Julius Brtko, Zdenek Dvorak

Abstract: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that controls the expression of a diverse set of genes. Structurally diverse compounds bind to AhR and act as AhR agonists. Well characterised family of natural AhR ligands are isoflavones, which are compounds found predominantly in soy beans or red clover. In this study we have examined agonistic effect of selected isoflavones (genistein, daidzein, biochanin A, formononetin and equol) on AhR in the novel transgenic gene reporter human cell line AZ-AhR, a stably transfected AhR-responsive cell line allowing rapid and sensitive assessment of AhR transcriptional activity. We demonstrated that biochanin A, formononetin and genistein at concentration 10–4 mol/l exerted agonistic effects on AhR with fold activation of 309-fold, 108-fold and 27-fold, which is about 84.8%, 29.6% and 7.4%, respectively, of the value attained by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Daidzein and equol did not show any significant effects on AhR.

Keywords: Isoflavones — Arylhydrocarbon receptor — AZ-AhR cells
Year: 2015, Volume: 34, Issue: 3 Page From: 331, Page To: 334

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