Home Endocrine Regulations 2015 Endocrine Regulations Vol.49, No.3, p.119–125, 2015

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Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

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Endocrine Regulations Vol.49, No.3, p.119–125, 2015

Title: Adding of ascorbic acid to the culture medium influences the antioxidant status and some biochemical parameters in the hen granulosa cells
Author: M. Capcarova, A. Kolesarova, A. Kalafova, J. Bulla, A. V. Sirotkin

Abstract: Objectives. The aim of the present study was to determine the activity of superoxide dismutase (SOD), total antioxidant status (TAS) of the hen granulosa cells, and selected biochemical parameters, including calcium, phosphorus, sodium, potassium, glucose, cholesterol, proteins, in the culture medium of granulosa cells after exposing them to ascorbic acid in vitro conditions.
Methods. Ovarian granulosa cells of hens were incubated with various doses of ascorbic acid (E1 0.09 mg/ml, E2 0.13 mg/ml, E3 0.17 mg/ml, E4 0.33 mg/ml, E5 0.5 mg/ml).
Results. Ascorbic acid did not manifest antioxidant potential and higher doses of ascorbic acid (0.17; 0.33 and 0.5 mg/ml) decreased the activity of SOD in granulosa cells. Vitamin application resulted in a significantly (p<0.05) higher accumulation of Na+ and K+ in culture media of granulosa cells and decreased the concentration of glucose and proteins.
Conclusion. These results indicate that ascorbic acid might be involved in the regulation of selected biochemical and physiological processes in ovarian granulosa cells.

Keywords: antioxidants, ascorbic acid, biochemistry, granulosa cells, hen
Published online: 03-Aug-2015
Year: 2015, Volume: 49, Issue: 3 Page From: 119, Page To: 125
Price: 15.40 €

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