Home Acta Virologica 2015 Acta Virologica Vol.59, No.3, p.209-220, 2015

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.59, No.3, p.209-220, 2015

Title: An amphipathic sequence in the cytoplasmic tail of HIV-1 Env alters cell tropism and modulates viral receptor specificity
Author: A. N. VZOROV, C. YANG, R. W. COMPANS

Abstract: The human immunodeficiency virus type 1 (HIV-1) 92UG046 Env protein, obtained from a CD4-independent HIV-1 primary isolate (Zerhouni et al., 2004), has the ability to initiate an infection in HeLa cells expressing CD4 when carrying the full-length (FL) Env, but uses CD8 molecules for receptor-mediated entry when carrying a truncated Env (CT84). To determine whether a specific length or structure in the cytoplasmic tail (CT) is responsible for this alteration of tropism, we compared a series of Env constructs with different CT truncations and the presence or absence of an amphipathic alpha- helical sequence. We found that truncated constructs containing the alpha-helical LLP-2 structure in their CT domains conferred a switch from CD4 to CD8 tropism. The results support the conclusion that the structure of the CT domain can play an important role in determining receptor specificity.

Keywords: HIV-1; Env glycoprotein; gp41; gp120; cytoplasmic tail truncation; alpha-helical LLP2 sequence; CD4, CD8; receptor specificity
Year: 2015, Volume: 59, Issue: 3 Page From: 209, Page To: 220
doi:10.4149/av_2015_03_209


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