Home Acta Virologica 2015 Acta Virologica Vol.59, No.3, p.247-256, 2015

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.59, No.3, p.247-256, 2015

Title: Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells
Author: A. Tarasevich, A. Filatov, A. Pichugin, D. Mazurov

Abstract: Human T lymphotropic virus 1 (HTLV-1) is a pathogenic retrovirus that spreads predominantly via cell-to-cell contact. Two models of cell-to-cell virus transmission are proposed: virological synapse (VS) and viral biofilms (VB). Both infectious structures can be involved in transmission and synergistically enhance HTLV-1 spread between cells. Although transmission of virus via VB has been reported, the molecular composition of VB remains poorly understood. In this study we generated new anti-VB monoclonal antibodies (MAbs) and screened
them along with a panel of anti-human cluster of differentiation (CD) MAbs to select antigens associated with VB. Among four MAbs generated against VB, two MAbs were identified as anti-CD25 (IL-2RA). We found that antigens CD4, CD150, CD25, CD70, and CD80 were enriched in VB. We also determined that expression of viral protein Tax, a central molecule in HTLV-1 transmission, upregulates intercellular adhesion molecule 1 (ICAM-1), CD95, CD25, CD70, and CD80. Whether these antigens are essential for VB formation and HTLV-1 infection remains unknown and will be determined in further experiments.

Keywords: monoclonal antibody; CD antigen; HTLV-1; biofilms
Year: 2015, Volume: 59, Issue: 3 Page From: 247, Page To: 256
doi:10.4149/av_2015_03_247


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