Acta Virologica Vol.59, No.3, p.295-299, 2015

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.59, No.3, p.295-299, 2015
Title: Preparation and identification of an antiserum against recombinant UL31 protein of pseudorabies virus
Author: M. S. CAI, S. JIANG, C. C. MO, J. L. WANG, J. L. HUANG, Z. C. ZENG, X. W. LI, Y. J. YANG, C. K. CHEN, W. CUI, M. L. LI

Abstract: Pseudorabies virus (PRV) late protein UL31 is a homologue of herpes simplex virus 1 (HSV-1) UL31, which is a multifunctional protein important for HSV-1 infection. However, the precise roles of PRV UL31 in virus life cycle are still poorly understood. A relatively crucial tool for uncovering the function of UL31 is an antiserum that specifically detects UL31 in the PRV-infected cells. For this purpose, a recombinant UL31 protein consisting of N-terminal 27 aa of UL31 fused to EYFP and His-tag was expressed, purified and used for the preparation of antiserum in BALB/c mice. Our results show that Western blot analysis and immunofluorescence assay showed that this antiserum could specifically detect the purified recombinant UL31 as well as full-length UL31 in the PRV infected cells. These results demonstrate that the prepared antiserum could serve as a valuable tool for further studies of UL31 functions in PRV infection.

Keywords: pseudorabies virus; UL31 protein; recombinant protein; antiserum

Year: 2015, Volume: 59, Issue: 3	Page From: 295, Page To: 299
doi:10.4149/av_2015_03_295
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