Neoplasma Vol.62, No.6, p.855-863, 2015
|
| Title: MicroRNA-219-5p functions as a tumor suppressor partially by targeting platelet-derived growth factor receptor alpha in colorectal cancer |
| Author: G. B. XIONG, G. N. ZHANG, Y. XIAO, B. Z. NIU, H. Z. QIU, B. WU, G. L. LIN, L. YOU, H. SHU |
|
Abstract: Platelet-derived growth factor receptor (PDGFR) signaling pathway was involved in the progress of colorectal cancer (CRC). By using the bioinformatic system online, we found that PDGFRα is a potential target of miR-219-5p. However, the expression pattern and underlying mechanisms of miR-219-5p had not been elucidated in CRC. Herein, we first evaluated the expression of miR-219-5p in tumor tissues by real-time polymerase chain reaction. Next, we confirmed that PDGFRα is the target of miR-219-5p by using luciferase report. And then, we investigated the biological functions of miR-219-5p in vitro in cell proliferation and apoptosis as well as cell cycle by gain and loss of function strategies. Data shown that miR-219-5p is down-regulated in CRC tissues compared with the corresponding matched normal tissues. PDGFRα was a direct target of miR-219-5p. Overexpression of miR-219-5p could inhibit cell proliferation, promote cell apoptosis and induce cell cycle arrest at the G1 phase. Furthermore, miR-219-5p suppressed the activation of the phosphatidylinositol 3-kinase/Akt signaling pathway and downregulated G1 cell-cycle-related protein cyclin D1, cyclin-dependent kinase (CDK) 4, and CDK6. Taken together, our results demonstrate that miR-219-5p functions as a tumor suppressor partially by targeting PDGFRα in colorectal cancer.
|
|
| Keywords: microRNA, colorectal cancer, miR-219-5p, PDGFRα, PI3K/Akt. |
|
|
Published online: 12-Oct-2015
|
| Year: 2015, Volume: 62, Issue: 6 |
Page From: 855, Page To: 863 |
doi:10.4149/neo_2015_104
|
|
 download file |
|
|
|
|
