Home Acta Virologica 2015 Acta Virologica Vol.59, No.4, p.398-404, 2015

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.59, No.4, p.398-404, 2015

Title: Sequence analysis and genetic diversity of five new Indian isolates of cucumber mosaic virus
Author: S. KUMAR, K. K. GAUTAM, S. K. RAJ

Abstract: Cucumber mosaic virus (CMV) is an important virus since it causes severe losses to many economically important crops worldwide. Five new isolates of CMV were isolated from naturally infected Hippeastrum hybridum, Dahlia pinnata, Hemerocallis fulva, Acorus calamus and Typhonium trilobatum plants, all exhibiting severe leaf mosaic symptoms. For molecular identification and sequence analyses, the complete coat protein (CP) gene of these isolates was amplified by RT-PCR. The resulting amplicons were cloned and sequenced and isolates were designated as HH (KP698590), DP (JF682239), HF (KP698589), AC (KP698588) and TT (JX570732). For study of genetic diversity among these isolates, the sequence data were analysed by BLASTn, multiple alignment and generating phylogenetic trees along with the respective sequences of other CMV isolates available in GenBank Database were done. The isolates under study showed 82–99% sequence diversity among them at nucleotide and amino acid levels; however they showed close relationships with CMV isolates of subgroup IB. In alignment analysis of amino acid sequences of HH and AC isolates, we have found fifteen and twelve unique substitutions, compared to HF, DP and TT isolates, suggesting the cause of high genetic diversity.

Keywords: cucumber mosaic virus; new isolates; capsid protein; genetic diversity
Published online: 06-Dec-2015
Year: 2015, Volume: 59, Issue: 4 Page From: 398, Page To: 404
doi:10.4149/av_2015_04_398


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