Home FOR AUTHORS General Physiology and Biophysics 2016 General Physiology and Biophysics Vol.35, No.2, p.155–164, 2016

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ISSN  1338-4325 (online)

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General Physiology and Biophysics Vol.35, No.2, p.155–164, 2016

Title: Sonodynamic therapy induces apoptosis of human leukemia HL-60 cells in the presence of protoporphyrin IX
Author: Xiaomin Su, Xiaobing Wang, Kun Zhang, Shuang Yang, Quanhong Liu, Albert W. Leung, Chuanshan Xu, Pan Wang
Abstract: Sonodynamic therapy (SDT) is expected to be a novel therapeutic strategy for tumor. The protoporphyrin IX disodium salt (PpIX), a photosensitizer, can be activated by ultrasound. The present study aims to investigate apoptosis of HL-60 cells induced by PpIX-mediated SDT. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was adopted to examine cell toxicity. Apoptosis was detected using Annexin V-PE/7-amino-actinomycin D (7-AAD) double staining. Detection of apoptotic bodies was examined by Hoechst33342 (HO) staining. Western blotting was used to analyze the protein of caspase-3 and poly ADP-ribose polymerase (PARP). Intracellular reactive oxygen species (ROS) was detected by a flow cytometer after exposures. Compared with PpIX alone and ultrasound alone groups, the synergistic cytotoxicity of PpIX plus ultrasound were significantly boosted. In addition, as determined by Annexin V-PE/7-AAD staining, SDT significantly induced HL-60 cell apoptosis, the obvious nuclear condensation was also found with HO staining at 4 hours post-SDT treatment. Furthermore, Western blotting showed visible enhancement of caspase-3 and PARP cleavage in this process. Besides, intracellular ROS production was significantly enhanced after SDT. Our findings demonstrate that PpIX-mediated SDT could induce apoptosis on HL-60 cells, suggesting that apoptosis is an important mechanism of cell death induced by PpIX-mediated SDT.
Keywords: Sonodynamic therapy — PpIX — Leukemia HL-60 cells — Apoptosis
Published online: 10-Mar-2016
Year: 2016, Volume: 35, Issue: 2 Page From: 155, Page To: 164
doi:10.4149/gpb_2015051


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