Home Acta Virologica 2016 Acta Virologica Vol.60, No.1, p.94-99, 2016

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.60, No.1, p.94-99, 2016

Title: Mapping of the regions involved in self-interaction of rice stripe virus P3 protein
Author: S. L. ZHAO, J. H. HAO, Y. N. XUE, C. Y. LIANG

Abstract: Rice stripe virus (RSV) protein P3 is a suppressor of RNA silencing in plants. P3 has been shown by biomolecular fluorescence complementation assay to self-interact in planta but the regions responsible for homotypic interaction have not been determined. Here we analyzed the domains for the self-interaction of P3 by using yeast two-hybrid, co-immunoprecipitation and fluorescence experiments. The results showed that P3 was also able to interact with itself in yeast and insect cells. The domain responsible for P3-P3 interaction was mapped to amino acids 15–30 at the N-terminal region of P3. Furthermore, subcellular localization suggested that the homo-oligomerization was the prerequisite for P3 to form larger protein aggregates in the nucleus of insect cell.

Keywords: rice stripe virus; P3; self-interaction; yeast two-hybrid; co-immunoprecipitation
Published online: 16-Mar-2016
Year: 2016, Volume: 60, Issue: 1 Page From: 94, Page To: 99
doi:10.4149/av_2016_01_94


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