Acta Virologica Vol.60, No.2, p.151-155, 2016

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.60, No.2, p.151-155, 2016
Title: Effect of the nucleotides surrounding the start codon on the translation of foot-and-mouth disease virus RNA
Author: X. X. MA, Y. P. FENG, Y. X. GU, J. H. ZHOU, Z. R. MA

Abstract: As for the alternative AUGs in foot-and-mouth disease virus (FMDV), nucleotide bias of the context flanking the AUG2nd could be used as a strong signal to initiate translation. To determine the role of the specific nucleotide context, dicistronic reporter constructs were engineered to contain different versions of nucleotide context linking between internal ribosome entry site (IRES) and downstream gene. The results indicate that under FMDV IRES-dependent mechanism, the nucleotide contexts flanking start codon can influence the translation initiation efficiencies. The most optimal sequences for both start codons have proved to be UUU AUG1st AAC and AAG AUG2nd GAA.

Keywords: foot-and-mouth disease virus; nucleotide bias; translation initiation; dicistronic reporter; internal ribosome entry site
	Published online: 06-Jun-2016
Year: 2016, Volume: 60, Issue: 2	Page From: 151, Page To: 155
doi:10.4149/av_2016_02_151
	download file

Aepress.sk