Home Acta Virologica 2016 Acta Virologica Vol.60, No.2, p.166-173, 2016

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.60, No.2, p.166-173, 2016

Title: Characterization of two anti-dengue human monoclonal antibodies prepared from PBMCs of patients with dengue illness in Thailand
Author: Z.-Y. LI, A. YAMASHITA, N. KAWASHITA, T. SASAKI, Y. PAN, K.-I. ONO, K. IKUTA, Y.-G. LI

Abstract: The global spread of the four dengue virus (DENV) serotypes (dengue-1 to -4) has made this virus a major and growing public health concern. Generally, pre-existing neutralizing antibodies derived from primary infection play a significant role in protecting against subsequent infection with the same serotype. By contrast, these pre-existing antibodies are believed to mediate a non-protective response to subsequent heterotypic DENV infections, leading to the onset of dengue illness. In this study, two monoclonal antibodies prepared by using peripheral blood mononuclear cells (PBMCs) from patients with dengue fever were characterized. Epitope mapping revealed that amino acid residues 254–278 in domain II of the viral envelope protein E were the target region of these antibodies. A database search revealed that certain sequences in this epitope region showed high conservation among the four serotypes of DENV. These two human monoclonal antibodies could neutralize DENV-2,-4 more effectively than DENV-1,-3. The amino acid sequences could not explain this difference in neutralizing activity. However, the 3D structure results showed that amino acid 274 could be the critical residue for the difference in neutralization. These results may provide basic information for the development of a dengue vaccine.

Keywords: human monoclonal antibody; epitope; dengue virus
Published online: 06-Jun-2016
Year: 2016, Volume: 60, Issue: 2 Page From: 166, Page To: 173
doi:10.4149/av_2016_02_166


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