Home Acta Virologica 2016 Acta Virologica Vol.60, No.2, p.190-195, 2016

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.60, No.2, p.190-195, 2016

Title: Downregulation of SWI5 and CTC1 genes: hepatitis B virus DNA polymerase transactivated protein 1-mediated inhibition of DNA repair
Author: X.-K. YAO, Z.-P. PAN, Y. LI, Y.-Z. LUN, Q. CHI, S.-J. JIANG, F. WANG, W. SUI

Abstract: Hepatitis B virus (HBV) DNA polymerase transactivated protein 1 (HBVDNAPTP1) is a novel protein upregulated by HBV DNA polymerase, which has been screened by suppression subtractive hybridization technique (SSH) (GenBank Acc. No. AY450389). A vector pcDNA3.1 (-)/myc-His A-HBVDNAPTP1 was constructed and used to transfect acute monocytic leukemia cell line THP-1. HBVDNAPTP1 expression was detected by Western blot analysis in the cells. A cDNA library of genes downregulated by HBVDNAPTP1 in THP-1 cells was made in pGEM-T Easy using SSH. The cDNAs were sequenced and analyzed with BLAST search against the sequences in GenBank. Some sequences, such as DNA repair protein SWI5 homolog (SWI5) and CTS telomere maintenance complex component 1 (CTC1), might be involved in DNA repair. Protein expression of SWI5 and CTC1 was identified by Western blot in THP-1 cells. HBVDNAPTP1 could downregulate the expression of SWI5 and CTC1 at translation level.

Keywords: hepatitis B virus; DNA polymerase; trans-regulation; suppression subtractive hybridization; SWI5; CTC1
Published online: 06-Jun-2016
Year: 2016, Volume: 60, Issue: 2 Page From: 190, Page To: 195

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