Home Acta Virologica 2016 Acta Virologica Vol.60, No.2, p.206-210, 2016

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.60, No.2, p.206-210, 2016

Title: New way of purification of pathogenic rickettsiae reducing health risks
Author: J. VALÁRIKOVÁ, Z. SEKEYOVÁ, L. ŠKULTÉTY, M. BOHÁCSOVÁ, M. QUEVEDO-DIAZ

Abstract: In general, cultivation and purification of intracellular pathogenic rickettsiae represents a risk for laboratory personnel due to exposure to highly infectious aerosol or accidental inoculation during these procedures. In this study, we describe an alternative, effective and time saving technique for rickettsial purification using digitonin to release intracellular bacteria from host cell without physical disruption. No significant differences were noted in yield and infectivity between digitonin treated rickettsiae and rickettsiae purified by sonication. This is the first report of using digitonin in purification of pathogenic rickettsiae and this approach might be effective for other intracellular pathogenic bacteria.

Keywords: Rickettsia akari; Rickettsia conorii; digitonin; purification
Published online: 06-Jun-2016
Year: 2016, Volume: 60, Issue: 2 Page From: 206, Page To: 210
doi:10.4149/av_2016_02_206


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