General Physiology and Biophysics Vol.35, No.3, p.387

General Physiology and Biophysics Vol.35, No.3, p.387–392, 2016

Founded: 1982
ISSN 1338-4325 (online)
ISSN 0231-5882 (print)
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General Physiology and Biophysics Vol.35, No.3, p.387–392, 2016
Title: A comparative study of protein patterns of human estrogen receptor positive (MCF-7) and negative (MDA-MB-231) breast cancer cell lines
Author: Dana Flodrova, Lucia Toporova, Dana Macejova, Marketa Lastovickova, Julius Brtko, Janette Bobalova

Abstract: In the present study, we analyzed the cell lysates of human tumour cell lines representing two major clinically different types of breast cancer. Our main goal was to show the differences between them on proteomic level. Gel electrophoresis followed by MALDI-TOF MS analysis was used for proteins determination. Exactly 98 proteins were unequivocally identified and 60 of them were expressed differentially between MDA-MB-231 and MCF-7 cell lines. Among the proteins reported here, some well-known breast cancer markers (e.g., annexin A1, annexin A2 and vimentin) were identified in the MDA-MB-231 cell line and thus we were able to distinguish both cell lines sufficiently.

Keywords: Cell line — Breast cancer — Mass spectrometry — Proteins
	Published online: 13-Jun-2016
Year: 2016, Volume: 35, Issue: 3	Page From: 387, Page To: 392
doi:10.4149/gpb_2016009
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