Neoplasma Vol.63, No.6, p.925-933,2016
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| Title: Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma |
| Author: U. ALTANEROVA, K. BENEJOVA, V. ALTANEROVA, S. TYCIAKOVA, B. RYCHLY, P. SZOMOLANYI, F. CIAMPOR, M. CIHOVA, V. REPISKA, K. ONDICOVA, B. MRAVEC, C. ALTANER |
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Abstract: We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is a drug approved for intravenous application to treat iron deficiency anemia in patients. Venofer labeling did not affect DP-MSCs or yCD::UPRT-DP-MSCs viability and growth kinetics. Electron microscopy of labeled cells showed internalized Venofer nanoparticles in endosomes. MRI relativity measurement of Venofer labeled DP-MSCs in a phantom arrangement revealed that 100 cells per 0.1 ml were still detectable. DP-MSCs or yCD::UPRT-DP-MSCs and the corresponding Venofer labeled cells release exosomes into conditional medium (CM). CM from yCD::UPRT-DP-MSCs in the presence of a prodrug 5-fluorocytosine caused tumor cell death in a dose dependent manner. Iron labeled DP-MSCs or yCD::UPRT-DP-MSCs sustained their tumor tropism in vivo; intra-nasally applied cells migrated and specifically engrafted orthotopic glioblastoma xenografts in rats.
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| Keywords: dental pulp MSCs, iron labeling, yCD::UPRT-exosomes, intranasal application, migration to intracerebral glioblastoma |
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Published online: 16-Nov-2016
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| Year: 2016, Volume: 63, Issue: 6 |
Page From: 925, Page To: 933 |
doi:10.4149/neo_2016_611
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