Home Acta Virologica 2016 Acta Virologica Vol.60, No.3, p.234-241, 2016

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.60, No.3, p.234-241, 2016

Title: Immunogenicity and antigenicity of a recombinant chimeric protein containing epitopes of poliovirus type 1
Author: X.-X. PAN, J. WANG, W.-Y. XIA, X.-F. LI, L.-J. YANG, C. HUANG, Y.-D. CHEN

Abstract: To design a vaccine that simultaneously prevents both rotavirus (RV) and poliovirus (PV), a PV type 1 (PV1) chimeric protein using RV VP6 as a vector (VP6F) was constructed, expressed in Escherichia coli expression system and characterized by SDS-PAGE, Western blot, immunofluorescence assay and neutralization test. The results showed that the chimeric protein reacted with anti-VP6F and anti-PV1 antibodies and elicited production of serum antibodies against the chimeric protein in guinea pigs. Antibodies against the chimeric protein neutralized RV Wa and PV1 infection in vitro. The results provided a relevant possibility of developing novel approaches in the rational design of vaccines effective against both RV and PV.

Keywords: chimeric protein; neutralizing activity; rotavirus; poliovirus epitope
Published online: 13-Sep-2016
Year: 2016, Volume: 60, Issue: 3 Page From: 234, Page To: 241
doi:10.4149/av_2016_03_234


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